Analysis of a human V beta gene subfamily

J Exp Med. 1986 Nov 1;164(5):1600-14. doi: 10.1084/jem.164.5.1600.

Abstract

We have isolated and sequenced five germline V beta gene segments that are homologous to the V region of the YT35 cDNA encoding the beta chain of the T cell antigen receptor from the tumor MOLT-3. One of these gene segments is identical to the YT35 V segment, and therefore is the corresponding germline V beta gene segment encoding the YT35 cDNA. The other four V beta members exhibit 77-98% homology to the YT35 V gene segment. Two of these V beta gene segments are pseudogenes. Analyses of the coding region sequences reveal that, although the V beta segments are very diverse, they are mutating at a rate comparable to that observed in most eukaryotic genes. Analyses of the genomic clones show that the spacing distance between germline V beta gene segments ranges from 3 kb to greater than 30 kb, and the entire V beta 8 subfamily appears to be linked by a total of no more than 110 kb of DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Biological Evolution
  • Chromosome Mapping
  • Codon
  • DNA / isolation & purification
  • Humans
  • Immunoglobulin Variable Region / genetics
  • Mutation
  • Nucleic Acid Hybridization
  • Receptors, Antigen, T-Cell / genetics*

Substances

  • Codon
  • Immunoglobulin Variable Region
  • Receptors, Antigen, T-Cell
  • DNA

Associated data

  • GENBANK/X06936
  • GENBANK/X07192
  • GENBANK/X07222
  • GENBANK/X07223
  • GENBANK/X07224
  • GENBANK/Y00349