Substrate specificity of DNA polymerases. II. 5-(1-Alkynyl)-dUTPs as substrates of the Klenow DNA polymerase enzyme

L Otvös; J Szécsi; J Sági; T Kovács

Substrate specificity of DNA polymerases. II. 5-(1-Alkynyl)-dUTPs as substrates of the Klenow DNA polymerase enzyme

Nucleic Acids Symp Ser. 1987:(18):125-9.

Authors

L Otvös¹, J Szécsi, J Sági, T Kovács

Affiliation

¹ Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.

PMID: 3697112

Abstract

A structure-activity relationship study was carried out with a sequence of 5-(1-alkynyl)-dUTPs in Klenow DNA polymerase enzyme-catalyzed reactions. Ability of modified dUTPs examined (up to the octynyl derivative) to serve as a substrate depended on both the length of substituent and the structure of DNA primer-template used.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA-Directed DNA Polymerase / metabolism*
Deoxyuracil Nucleotides* / chemical synthesis*
Kinetics
Substrate Specificity

Substances

Deoxyuracil Nucleotides
deoxyuridine triphosphate
DNA-Directed DNA Polymerase