Abstract
An in vitro system was used to study DNA synthesis in lysates of Escherichia coli cells which had been grown in the presence of ethionine. Such lysates showed a reduced capacity to incorporate [3H]TTP into high-molecular-weight material. Activity could be restored by incubation with S-adenosyl methionine and ATP. S-adenosyl methionine-reactivated TTP incorporation required the presence of DNA polymerase I, ATP, and all four deoxyribonucleotide triphosphates. DNA polymerase III was not required.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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Chloramphenicol / pharmacology
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DNA / biosynthesis*
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DNA Polymerase I / metabolism*
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DNA-Directed DNA Polymerase / metabolism*
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Deoxyribonucleotides / metabolism
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Escherichia coli / metabolism*
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Ethionine / pharmacology
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Methylation
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S-Adenosylmethionine / metabolism*
Substances
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Deoxyribonucleotides
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Chloramphenicol
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S-Adenosylmethionine
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Adenosine Triphosphate
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DNA
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DNA Polymerase I
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DNA-Directed DNA Polymerase
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Ethionine