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Conditionals by inversion provide a universal method for the generation of conditional alleles.
Economides AN, Frendewey D, Yang P, Dominguez MG, Dore AT, Lobov IB, Persaud T, Rojas J, McClain J, Lengyel P, Droguett G, Chernomorsky R, Stevens S, Auerbach W, Dechiara TM, Pouyemirou W, Cruz JM Jr, Feeley K, Mellis IA, Yasenchack J, Hatsell SJ, Xie L, Latres E, Huang L, Zhang Y, Pefanis E, Skokos D, Deckelbaum RA, Croll SD, Davis S, Valenzuela DM, Gale NW, Murphy AJ, Yancopoulos GD. Economides AN, et al. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88. doi: 10.1073/pnas.1217812110. Epub 2013 Aug 5. Proc Natl Acad Sci U S A. 2013. PMID: 23918385 Free PMC article.
Targeted deletion of the sclerostin gene in mice results in increased bone formation and bone strength.
Li X, Ominsky MS, Niu QT, Sun N, Daugherty B, D'Agostin D, Kurahara C, Gao Y, Cao J, Gong J, Asuncion F, Barrero M, Warmington K, Dwyer D, Stolina M, Morony S, Sarosi I, Kostenuik PJ, Lacey DL, Simonet WS, Ke HZ, Paszty C. Li X, et al. J Bone Miner Res. 2008 Jun;23(6):860-9. doi: 10.1359/jbmr.080216. J Bone Miner Res. 2008. PMID: 18269310 Free article.
Sclerostin expression in trabecular bone is downregulated by osteoclasts.
Koide M, Yamashita T, Murakami K, Uehara S, Nakamura K, Nakamura M, Matsushita M, Ara T, Yasuda H, Penninger JM, Takahashi N, Udagawa N, Kobayashi Y. Koide M, et al. Sci Rep. 2020 Aug 13;10(1):13751. doi: 10.1038/s41598-020-70817-1. Sci Rep. 2020. PMID: 32792620 Free PMC article.
A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ. Tang T, et al. Nat Biotechnol. 2010 Jul;28(7):749-55. doi: 10.1038/nbt.1644. Epub 2010 Jun 20. Nat Biotechnol. 2010. PMID: 20562862