A long non-coding RNA as a direct vitamin D target transcribed from the antisense strand of the human HSD17B2 locus

Yoshiaki Kanemoto; Koichi Nishimura; Akira Hayakawa; Takahiro Sawada; Rei Amano; Jinichi Mori; Tomohiro Kurokawa; Yoshinori Murakami; Shigeaki Kato

doi:10.1042/BSR20220321

A long non-coding RNA as a direct vitamin D target transcribed from the antisense strand of the human HSD17B2 locus

Biosci Rep. 2022 May 27;42(5):BSR20220321. doi: 10.1042/BSR20220321.

Authors

Yoshiaki Kanemoto^{1

2

3}, Koichi Nishimura^{1

2}, Akira Hayakawa^{1

2}, Takahiro Sawada^{1

2}, Rei Amano^{1

2}, Jinichi Mori^{1

2

4

5}, Tomohiro Kurokawa^{1

2

4}, Yoshinori Murakami³, Shigeaki Kato^{1

2

4}

Affiliations

¹ Department of Medical Epigenetics, Research Institute of Innovative Medicine, Tokiwa Foundation, Iwaki, Fukushima, Japan.
² Department of Medical Genome Research, Graduate School of Life Science and Engineering, Iryo Sosei University, Iino, Chuo-dai, Iwaki, Fukushima 9708551, Japan.
³ Division of Molecular Pathology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
⁴ Department of Basic Pathology, School of Medicine, Fukushima Medical University, Fukushima, Fukushima 960-1295, Japan.
⁵ Department of Hematology, Jyoban Hospital, Tokiwa Foundation, Iwaki, Fukushima, Japan.

Abstract

Vitamin D (VD) exerts a wide variety of actions via gene regulation mediated by the nuclear vitamin D receptor (VDR) under physiological and pathological settings. However, the known target genes of VDR appear unlikely to account for all VD actions. We used in silico and transcriptomic approaches in human cell lines to search for non-coding RNAs transcriptionally regulated by VD directly. Four long non-coding RNAs (lncRNAs), but no microRNAs (miRNAs), were found, supported by the presence of consensus VDR-binding motifs in the coding regions. One of these lncRNAs (AS-HSD17β2) is transcribed from the antisense strand of the HSD17β2 locus, which is also a direct VD target. AS-HSD17β2 attenuated HSD17β2 expression. Thus, AS-HSD17β2 represents a direct lncRNA target of VD.

Keywords: VDR; eukaryotic gene expression; lncRNA; non-coding RNA; vitamin D.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Estradiol Dehydrogenases
Humans
MicroRNAs* / metabolism
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
Vitamin D / genetics
Vitamin D / pharmacology
Vitamins

Substances

MicroRNAs
RNA, Long Noncoding
Vitamins
Vitamin D
Estradiol Dehydrogenases
HSD17B2 protein, human