Mutations of MSH5 in nonobstructive azoospermia (NOA) and rescued via in vivo gene editing

Min Chen; Chencheng Yao; Yingying Qin; Xiuhong Cui; Peng Li; Zhiyong Ji; Limei Lin; Haowei Wu; Zhi Zhou; Yaoting Gui; Zheng Li; Fei Gao

doi:10.1038/s41392-021-00710-4

Mutations of MSH5 in nonobstructive azoospermia (NOA) and rescued via in vivo gene editing

Signal Transduct Target Ther. 2022 Jan 3;7(1):1. doi: 10.1038/s41392-021-00710-4.

Authors

Min Chen^#¹, Chencheng Yao^#², Yingying Qin^#³, Xiuhong Cui⁴, Peng Li², Zhiyong Ji², Limei Lin^{4

5

6}, Haowei Wu^{4

5

6}, Zhi Zhou⁷, Yaoting Gui⁸, Zheng Li⁹, Fei Gao^{10

11

12}

Affiliations

¹ Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, P. R. China.
² Department of Andrology, the Center for Men's Health, Urologic Medical Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China.
³ Center for Reproductive Medicine of Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, P. R. China.
⁴ State Key Laboratory of Stem cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, P. R. China.
⁵ University of Chinese Academy of Sciences, Beijing, P. R. China.
⁶ Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
⁷ School of Life Science and Technology, ShanghaiTech University, Shanghai, P. R. China.
⁸ Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, P. R. China. guiyaoting2007@aliyun.com.
⁹ Department of Andrology, the Center for Men's Health, Urologic Medical Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China. lizhengboshi@163.com.
¹⁰ State Key Laboratory of Stem cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, P. R. China. gaof@ioz.ac.cn.
¹¹ University of Chinese Academy of Sciences, Beijing, P. R. China. gaof@ioz.ac.cn.
¹² Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. gaof@ioz.ac.cn.

^# Contributed equally.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Azoospermia* / genetics
Azoospermia* / metabolism
Azoospermia* / therapy
CRISPR-Cas Systems*
Cell Cycle Proteins* / genetics
Cell Cycle Proteins* / metabolism
DNA-Binding Proteins* / genetics
DNA-Binding Proteins* / metabolism
Gene Editing*
Humans
Male
Mice
Mice, Mutant Strains

Substances

Cell Cycle Proteins
DNA-Binding Proteins
MSH5 protein, human
Msh5 protein, mouse

Supplementary concepts

Azoospermia, Nonobstructive