Abstract
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Genes
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Genes, Viral*
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Genetic Vectors
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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T-Phages / enzymology
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T-Phages / genetics*
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Viral Proteins / biosynthesis
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Viral Proteins / genetics*
Substances
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Recombinant Fusion Proteins
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Viral Proteins