Objectives: To study the value of serum miR-922 and miR-506 expression levels in the diagnosis and prognostic assessment of childhood acute lymphoblastic leukemia (ALL).
Methods: A total of 132 children with ALL (ALL group) and 80 healthy children (healthy control group) were prospectively selected in this study. Quantitative real-time polymerase chain reaction was used to measure the expression levels of serum miR-922 and miR-506 in both groups. Receiver operating characteristic (ROC) curves were plotted to analyze the diagnostic value of miR-922 and miR-506 for childhood ALL. The Kaplan-Meier method was used to plot survival curves, and multivariate COX regression models were used to analyze the risk factors for poor prognosis in children with ALL.
Results: The ALL group had significantly higher expression levels of serum miR-922 and miR-506 than the control group (P<0.001). The ROC curve analysis showed that the optimal cut-off values of miR-922 and miR-506 for the diagnosis of childhood ALL were 1.46 and 2.17, respectively. The high miR-922 expression (≥1.46) group and high miR-506 expression (≥2.17) group had significantly higher incidence rates of lymph node enlargement, leukocyte count ≥50×109/L, medium-high risk stratification, mixed-lineage leukemia (MLL) gene rearrangement, and karyotype abnormality than the low miR-922 expression group and low miR-506 expression group (P<0.05). The Kaplan-Meier analysis showed that high expression of miR-922 and miR-506 was associated with short survival time in children with ALL (P<0.05). The multivariate COX regression analysis showed that leukocyte count ≥50×109/L, medium-high risk stratification, MLL gene rearrangement, miR-922≥1.46, and miR-506≥2.17 could indicate poor prognosis in children with ALL (P<0.05).
Conclusions: The expression levels of miR-922 and miR-506 are of good value in the diagnosis and prognostic assessment of childhood ALL.
目的: 探讨血清miR-922及miR-506表达水平对儿童急性淋巴细胞白血病（acute lymphoblastic leukemia，ALL）诊断及预后判断的价值。方法: 前瞻性选取132例ALL患儿（ALL组）和80例健康儿童（健康对照组）纳入本研究，采用实时荧光定量PCR技术检测ALL组和健康对照组血清miR-922及miR-506表达水平。绘制受试者工作特征曲线分析miR-922及miR-506对儿童ALL的诊断价值。应用Kaplan-Meier法绘制生存曲线，采用多因素COX回归模型分析影响ALL患儿预后不良的因素。结果: ALL组血清miR-922及miR-506表达水平均明显高于健康对照组（P<0.001）。受试者工作特征曲线分析结果显示，miR-922及miR-506诊断儿童ALL的最佳截断值分别为1.46、2.17。miR-922高表达组（≥1.46）及miR-506高表达组（≥2.17）的淋巴结肿大、白细胞计数≥50×109/L、中高危险度分层、MLL基因重排、染色体核型异常的发生率均明显高于miR-922及miR-506低表达组（P<0.05）。Kaplan-Meier分析结果显示，miR-922及miR-506高表达与ALL患儿生存期短有关（P<0.05）。多因素COX回归分析结果显示，白细胞计数≥50×109/L、危险度分层为中高危、MLL基因重排、miR-922≥1.46及miR-506≥2.17可提示ALL患儿预后不良（P<0.05）。结论: miR-922及miR-506表达水平对儿童ALL诊断及预后判断均有较好的价值。.
Keywords: Acute lymphoblastic leukemia; Child; Diagnostic value; Prognosis; miR-506; miR-922.