Mapping covariance in brain FDG uptake to structural connectivity

Purpose: Inter-subject covariance of regional 18F-fluorodeoxyglucose (FDG) PET measures (FDG_cov) as proxy of brain connectivity has been gaining an increasing acceptance in the community. Yet, it is still unclear to what extent FDG_cov is underlied by actual structural connectivity via white matter fiber tracts. In this study, we quantified the degree of spatial overlap between FDG_cov and structural connectivity networks.

Methods: We retrospectively analyzed neuroimaging data from 303 subjects, both patients with suspected neurodegenerative disorders and healthy individuals. For each subject, structural magnetic resonance, diffusion tensor imaging, and FDG-PET data were available. The images were spatially normalized to a standard space and segmented into 62 anatomical regions using a probabilistic atlas. Sparse inverse covariance estimation was employed to estimate FDG_cov. Structural connectivity was measured by streamline tractography through fiber assignment by continuous tracking.

Results: For the whole brain, 55% of detected connections were found to be convergent, i.e., present in both FDG_cov and structural networks. This metric for random networks was significantly lower, i.e., 12%. Convergent were 80% of intralobe connections and only 30% of interhemispheric interlobe connections.

Conclusion: Structural connectivity via white matter fiber tracts is a relevant substrate of FDG_cov, underlying around a half of connections at the whole brain level. Short-range white matter tracts appear to be a major substrate of intralobe FDG_cov connections.