Objective: To investigate the expression and clinical significance of serum protein ROCK2 in patients with chronic graft-versus-host disease (cGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).
Methods: The patients were divided into cGVHD group and control group (without cGVHD). The expression levels of serum protein ROCK2 were detected by ELISA in patients with or without cGVHD after allo-HSCT.
Results: The expression level of ROCK2 in serum of cGVHD patients was significantly higher than those in control group, moreover, the expression level of ROCK2 in severe cGVHD group was significant higher than that in moderate and mild cGVHD group (P<0.001). The expression level of ROCK2 was significantly decreased in the serum of cGVHD patients after treatment（P＜0.01）; the expression level of ROCK2 was significantly higher in the serum of cGVHD patients with lung as the target organ（P＜0.01）. The median survival time of patients with severe cGVHD were significantly shorter than that of patients with mild and moderate cGVHD（P＜0.05）.
Conclusion: ROCK2 shows certain reference value in the evaluation of severity and prognosis of cGVHD, and may be a new target for the treatment of cGVHD.
题目: 血清蛋白ROCK2在慢性移植物抗宿主病患者中的表达及意义.
目的: 探讨异基因造血干细胞移植后患者血清蛋白ROCK2在慢性移植物抗宿主病（cGVHD）患者中的表达及临床意义.
方法: 将患者分为cGVHD组和对照组（未发生cGVHD），采用ELISA法检测接受异基因造血干细胞移植后发生和未发生cGVHD 患者血清蛋白ROCK2的表达水平.
结果: cGVHD患者血清中ROCK2表达水平较对照组显著升高（P＜0.05），其中ROCK2在重度cGVHD组表达水平较轻、中度cGVHD组明显升高（P＜0.001）；ROCK2表达水平在发生cGVHD患者治疗后较治疗前明显下降（P＜0.01）。在以肺脏为主要靶器官的cGVHD患者血清中ROCK2显著升高（P＜0.01）。发生重度cGVHD的患者，中位生存时间较轻度和中度cGVHD患者明显缩短（P＜0.05）.
结论: ROCK2对评估cGVHD严重程度和预后有一定参考价值，可能成为治疗cGVHD的新靶标.