Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato

J Bacteriol. 1988 Jun;170(6):2879-83. doi: 10.1128/jb.170.6.2879-2883.1988.

Abstract

The nucleotide sequence of a 4.5-kilobase copper resistance determinant from Pseudomonas syringae pv. tomato revealed four open reading frames (ORFs) in the same orientation. Deletion and site-specific mutational analyses indicated that the first two ORFs were essential for copper resistance; the last two ORFs were required for full resistance, but low-level resistance could be conferred in their absence. Five highly conserved, direct 24-base repeats were found near the beginning of the second ORF, and a similar, but less conserved, repeated region was found in the middle of the first ORF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Deletion
  • Copper / pharmacology*
  • Drug Resistance, Microbial
  • Genes*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Mutation
  • Pseudomonas / drug effects
  • Pseudomonas / genetics*

Substances

  • Copper

Associated data

  • GENBANK/M19930