Sites of termination of in vitro DNA synthesis on cis-diamminedichloroplatinum(II) treated single-stranded DNA: a comparison between E. coli DNA polymerase I and eucaryotic DNA polymerases alpha

Nucleic Acids Res. 1988 May 25;16(10):4407-18. doi: 10.1093/nar/16.10.4407.

Abstract

We have compared the capacity of the large fragment of E. coli DNA polymerase I and highly purified DNA polymerases alpha from either Drosophila melanogaster embryos or calf thymus to replicate single-stranded M13 mp10 DNA treated with the antitumoral drug cis-diamminedichloroplatinum(II) (cis-DDP). We report that: a) although prokaryotic and eukaryotic enzymes have different structural complexity and dissimilar in vivo functions, their synthesis was blocked in vitro at similar sites on cis-DDP treated DNA; b) this inhibition occurred not only at d(G)n sequences, as previously reported for E. coli DNA polymerase I, (Pinto & Lippard (1985) Proc. Natl. Acad. Sci. USA, 82, 4616-4619) but also at other sequences which may represent putative cis-DDP-DNA adducts.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • Cisplatin / pharmacology*
  • DNA Damage
  • DNA Polymerase I / metabolism*
  • DNA Polymerase II / metabolism*
  • DNA Replication / drug effects*
  • DNA, Single-Stranded / drug effects
  • DNA, Single-Stranded / genetics*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology
  • Embryo, Nonmammalian / enzymology
  • Escherichia coli / enzymology*
  • Molecular Sequence Data
  • Templates, Genetic
  • Thymus Gland / enzymology

Substances

  • DNA, Single-Stranded
  • DNA Polymerase I
  • DNA Polymerase II
  • Cisplatin