The long noncoding RNA ST7-AS1 promotes laryngeal squamous cell carcinoma by stabilizing CARM1

Haiping Qin; Jinxia Xu; Lili Gong; Baolu Jiang; Wei Zhao

doi:10.1016/j.bbrc.2019.02.057

The long noncoding RNA ST7-AS1 promotes laryngeal squamous cell carcinoma by stabilizing CARM1

Biochem Biophys Res Commun. 2019 Apr 23;512(1):34-40. doi: 10.1016/j.bbrc.2019.02.057. Epub 2019 Mar 7.

Authors

Haiping Qin¹, Jinxia Xu², Lili Gong², Baolu Jiang², Wei Zhao²

Affiliations

¹ Department of Otolaryngology, Head and Neck Surgery, Liaocheng People's Hospital, Liaocheng, 252000, Shandong, China. Electronic address: hpqin_lph@yeah.net.
² Department of Otolaryngology, Head and Neck Surgery, Liaocheng People's Hospital, Liaocheng, 252000, Shandong, China.

PMID: 30853182
DOI: 10.1016/j.bbrc.2019.02.057

Abstract

The laryngeal squamous cell carcinoma (LSCC) represents a malignant cancer and contributes largely to head and neck tumorigenesis. The molecular mechanisms for LSCC progression are poorly understood. In current work, we identified long non-coding RNA (lncRNA) termed suppressor of tumorigenicity 7 antisense RNA 1 (ST7-AS1) as an oncogenic factor in LSCC. ST7-AS1 is frequently overexpressed in LSCC tissues and cell lines. ST7-AS1 is required for the malignancy of LSCC cells through migration, tumor sphere formation assay and in vivo implantation. Mechanistically, ST7-AS1 could interact with CARM1, a well characterized protein arginine methyltransferase and protect CARM1 from ubiquitin-dependent degradation. CARM1 can methylate Sox-2, a pluripotent transcription factor. Thus, ST7-AS1 might mediate its oncogenic effect by signaling through CARM1-Sox-2 axis to enhance Sox-2 self-association and transactivation activity. Collectively, we have unraveled a ST7-AS1/CARM1/Sox-2 signaling axis in LSCC and may have created novel interconnections between noncoding RNAs and cancer development.

Keywords: CARM1; Laryngeal squamous cell carcinoma; ST7-AS1; Sox-2; lncRNA.

MeSH terms

Carcinogenesis / genetics
Carcinogens / metabolism
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / metabolism*
Cell Line, Tumor
Disease Progression
Enzyme Stability / genetics
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Laryngeal Neoplasms / genetics*
Laryngeal Neoplasms / metabolism*
Proteasome Endopeptidase Complex / metabolism
Protein-Arginine N-Methyltransferases / metabolism*
Proteolysis
RNA, Antisense / genetics
RNA, Antisense / metabolism
RNA, Long Noncoding / genetics*
RNA, Long Noncoding / metabolism*
SOXB1 Transcription Factors / metabolism
Tumor Suppressor Proteins / genetics

Substances

Carcinogens
RNA, Antisense
RNA, Long Noncoding
SOX2 protein, human
SOXB1 Transcription Factors
ST7 protein, human
Tumor Suppressor Proteins
Protein-Arginine N-Methyltransferases
coactivator-associated arginine methyltransferase 1
Proteasome Endopeptidase Complex