Abstract
Complementary DNA clones of a putative transforming gene were isolated from NIH 3T3 cells transformed with human Ewing sarcoma DNA. The gene was termed B-raf because it is related to but distinct from c-raf and A-raf. It appears that substitution in the amino-terminal portion of the normal B-raf protein confers transforming activity to the gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Line, Transformed
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Cloning, Molecular
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DNA / analysis*
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DNA / metabolism
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Gene Expression Regulation*
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Humans
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Molecular Sequence Data
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Protein Kinases / genetics
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-raf
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Sarcoma, Ewing / analysis
Substances
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Proto-Oncogene Proteins
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DNA
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Protein Kinases
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Proto-Oncogene Proteins c-raf