Association of Epstein-Barr virus early antigen diffuse component and virus-specified DNA polymerase activity

J Virol. 1987 Sep;61(9):2947-9. doi: 10.1128/JVI.61.9.2947-2949.1987.

Abstract

The role of Epstein-Barr virus (EBV) early antigen diffuse component (EA-D) and its relationship with EBV DNA polymerase in EBV genome-carrying cells are unclear, EBV-specified DNA polymerase was purified in a sequential manner from Raji cells treated with phorbol-12,13-dibutyrate and n-butyrate by phosphocellulose, DEAE-cellulose, double-stranded DNA-cellulose, and blue Sepharose column chromatography. Four polypeptides with molecular masses of 110,000, 100,000, 55,000, and 49,000 daltons were found to be associated with EBV-specified DNA polymerase activity. A monoclonal antibody which could neutralize the EBV DNA polymerase activity was prepared and found to recognize 55,000- and 49,000-dalton polypeptides. An EA-D monoclonal antibody, R3 (G. R. Pearson, V. Vorman, B. Chase, T. Sculley, M. Hummel, and E. Kieff, J. Virol. 47:183-201, 1983), was also able to recognize these same two polypeptides associated with EBV DNA polymerase activity. It was concluded that EBV EA-D polypeptides, as identified by R3 monoclonal antibody, are critical components of EBV DNA polymerase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, Viral / analysis*
  • Antigens, Viral / immunology
  • DNA-Directed DNA Polymerase / analysis*
  • DNA-Directed DNA Polymerase / isolation & purification
  • Herpesvirus 4, Human / enzymology*
  • Molecular Weight

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral
  • Epstein-Barr virus early antigen
  • DNA-Directed DNA Polymerase