Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene

Science. 1987 Jun 5;236(4806):1308-11. doi: 10.1126/science.3035717.

Abstract

Cell cycle-dependent histone genes are transcribed at a basal level throughout the cell cycle, with a three- to fivefold increase during early S phase. Protein-DNA interactions in the 5' promoter region of a cell cycle-regulated human H4 histone gene have been analyzed at single-nucleotide resolution in vivo. This region contains two sites, with four potential protein-binding domains, at which the DNA is protected from reaction with dimethyl sulfate in cells and from digestion with deoxyribonuclease I in nuclei. These protein-DNA interactions persist during all phases of the cell cycle and dissociate with 0.16 to 0.2M sodium chloride.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cell Cycle
  • Cell Line
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease I
  • Gene Expression Regulation
  • Histones / genetics*
  • Humans
  • Nucleic Acid Hybridization
  • Promoter Regions, Genetic*
  • Protein Binding
  • Sulfuric Acid Esters

Substances

  • Histones
  • Sulfuric Acid Esters
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease I
  • dimethyl sulfate

Associated data

  • GENBANK/M16707