Initiation of phage phi 29 DNA replication by mutants with deletions at the carboxyl end of the terminal protein

Gene. 1986;43(1-2):103-10. doi: 10.1016/0378-1119(86)90013-2.

Abstract

Series of deletions at the C end of p3, the phage phi 29 DNA terminal protein (TP), have been constructed and characterized. Measurements of the activity of those deletion mutants in the formation of the p3-dAMP initiation complex in vitro indicate the need of an intact C-end for the normal TP primer function in DNA replication. It appears that the region at the C-end between aa 240 and 262 of p3, or part of it, might be essential for the normal TP function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromosome Deletion*
  • Coliphages / genetics*
  • DNA Replication*
  • DNA Restriction Enzymes
  • Escherichia coli / genetics*
  • Genes
  • Genes, Viral
  • Mutation*
  • Plasmids
  • Viral Proteins / genetics*

Substances

  • Viral Proteins
  • terminal protein, Bacillus phage phi29
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J02479
  • GENBANK/M13659