Poly(dG-dC) in the Z-form inhibits E. coli DNA polymerase I and AMV DNA polymerase activity

Biochem Int. 1985 Sep;11(3):281-90.

Abstract

The effect of Z-conformation of DNA on its template activity in DNA synthesis reactions in vitro has been studied. Normal poly(dG-dC) in the B-form, brominated and unbrominated in the Z-form have been compared for their template activity in DNA synthesis reactions mediated by AMV DNA polymerase and E. coli DNA polymerase I. The results indicate that poly(dG-dC) in the Z-form is totally inactive as a template for DNA synthesis and further that it is a strong competitive inhibitor of copying of the B-form DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Avian Leukosis Virus / enzymology*
  • Avian Myeloblastosis Virus / enzymology*
  • Circular Dichroism
  • DNA Polymerase I / antagonists & inhibitors*
  • Escherichia coli / enzymology*
  • Kinetics
  • Nucleic Acid Conformation
  • Nucleic Acid Synthesis Inhibitors*
  • Polydeoxyribonucleotides / metabolism*
  • Templates, Genetic

Substances

  • Nucleic Acid Synthesis Inhibitors
  • Polydeoxyribonucleotides
  • poly(dC-dG)
  • DNA Polymerase I