Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon

J Biol Chem. 1985 May 10;260(9):5616-20.

Abstract

The preceding paper presents evidence for the co-transcriptional expression of the ileS and lsp genes in Escherichia coli. To identify the promoter for the ileS-lsp operon, we have determined the nucleotide sequence of an 1.8-kilobase DNA fragment between the rpsT and IleS genes. The sequence data have revealed an open reading frame, designated gene X, which encodes a polypeptide with 312 amino acid residues. Both in vivo and in vitro expressions of the x gene result in the synthesis of a soluble protein with an apparent Mr of 35,000. The x gene is transcribed in the same direction as that of the ileS-lsp operon and opposite to that of the upstream adjacent rpsT gene. No transcription termination sequence can be discerned in the intercistronic region between the x and ileS genes. DNase I footprinting experiment revealed a RNA polymerase binding site at 170-151 base pairs upstream of the x gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / analysis
  • Base Sequence
  • DNA Restriction Enzymes / metabolism
  • DNA-Directed RNA Polymerases / metabolism
  • Deoxyribonuclease HindIII
  • Deoxyribonucleases, Type II Site-Specific*
  • Escherichia coli / genetics*
  • Gene Expression Regulation
  • Operon*
  • Plasmids

Substances

  • Bacterial Proteins
  • DNA-Directed RNA Polymerases
  • DNA Restriction Enzymes
  • Deoxyribonuclease HindIII
  • endodeoxyribonuclease HpaI
  • Deoxyribonucleases, Type II Site-Specific

Associated data

  • GENBANK/M10428