Abstract
The role of JAK/STAT3-mediated signaling pathway in the realization of the growth potential of mesenchymal precursor cells was examined in vitro. The stimulating role of JAKs and STAT3 towards proliferating activity of progenitor cells and their different role in the regulation of differentiation of the progenitor elements were demonstrated. Inhibitors of JAKs and STAT3 reduced the yield of fibroblast CFU and their mitotic activity. Blockade of JAKs accelerated and selective inactivation of STAT3 decelerated differentiation of progenitor cells.
Keywords:
JAK; STAT; progenitor cells; regenerative medicine; signal transduction.
MeSH terms
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Animals
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Anthraquinones / pharmacology
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Bone Marrow Cells / cytology
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / metabolism*
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Cell Differentiation / drug effects
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Cell Proliferation / drug effects
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism*
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Gene Expression Regulation
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Janus Kinase 1 / antagonists & inhibitors
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Janus Kinase 1 / genetics*
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Janus Kinase 1 / metabolism
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Janus Kinase 3 / antagonists & inhibitors
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Janus Kinase 3 / genetics*
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Janus Kinase 3 / metabolism
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / metabolism*
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Mice
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Mice, Inbred C57BL
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Nitriles
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Phosphorylation
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Primary Cell Culture
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Pyrazoles / pharmacology
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Pyrimidines
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STAT3 Transcription Factor / antagonists & inhibitors
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STAT3 Transcription Factor / genetics*
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STAT3 Transcription Factor / metabolism
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Signal Transduction
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Sulfonamides / pharmacology
Substances
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Anthraquinones
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LLL12 compound
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Nitriles
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Pyrazoles
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Pyrimidines
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STAT3 Transcription Factor
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Stat3 protein, mouse
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Sulfonamides
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ruxolitinib
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Jak1 protein, mouse
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Jak3 protein, mouse
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Janus Kinase 1
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Janus Kinase 3