Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ

Sonali S Vishal; Sarika Tilwani; Sorab N Dalal

doi:10.1016/j.bbrc.2017.12.080

Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ

Biochem Biophys Res Commun. 2018 Jan 8;495(2):1998-2003. doi: 10.1016/j.bbrc.2017.12.080. Epub 2017 Dec 15.

Authors

Sonali S Vishal¹, Sarika Tilwani¹, Sorab N Dalal²

Affiliations

¹ KS215, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar Node, Navi Mumbai 410210, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400085, India.
² KS215, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar Node, Navi Mumbai 410210, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400085, India. Electronic address: sdalal@actrec.gov.in.

PMID: 29253567
DOI: 10.1016/j.bbrc.2017.12.080

Abstract

Desmosomes are cell-cell adhesion junctions that anchor intermediate filaments. Loss of 14-3-3γ in HCT116 cells led to defects in desmosome assembly due to a decrease in the transport of Plakoglobin (PG) to the cell border thus disrupting desmosome formation. Desmosome formation in cells lacking 14-3-3γ was restored by artificially localizing PG to the cell border by fusing it to EGFP-f (PG-EGFP-f). These results suggest that a major role of 14-3-3γ in desmosome assembly is to transport PG to the cell border leading to the initiation of desmosome formation.

Keywords: 14-3-3γ; Cell-cell adhesion; Desmosome; Plakoglobin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

14-3-3 Proteins / metabolism*
Cell Line, Tumor
Cell Membrane / metabolism*
Colorectal Neoplasms / metabolism*
Desmosomes / metabolism*
Humans
Subcellular Fractions / metabolism*
gamma Catenin / metabolism

Substances

14-3-3 Proteins
JUP protein, human
gamma Catenin