Nuclear myosin 1 associates with papillomavirus E2 regulatory protein and influences viral replication

Eve Sankovski; Aare Abroi; Mart Ustav Jr; Mart Ustav

doi:10.1016/j.virol.2017.11.013

Nuclear myosin 1 associates with papillomavirus E2 regulatory protein and influences viral replication

Virology. 2018 Jan 15:514:142-155. doi: 10.1016/j.virol.2017.11.013. Epub 2018 Jan 4.

Authors

Eve Sankovski¹, Aare Abroi², Mart Ustav Jr³, Mart Ustav⁴

Affiliations

¹ University of Tartu, Institute of Technology, Nooruse 1, 50411 Tartu, Estonia.
² Estonian Biocentre, Riia 23, 51010 Tartu, Estonia.
³ University of Tartu, Institute of Technology, Nooruse 1, 50411 Tartu, Estonia; Icosagen Cell Factory OÜ, Eerika tee 1, Õssu küla, Ülenurme vald, 61713 Tartumaa, Estonia.
⁴ University of Tartu, Institute of Technology, Nooruse 1, 50411 Tartu, Estonia; Icosagen Cell Factory OÜ, Eerika tee 1, Õssu küla, Ülenurme vald, 61713 Tartumaa, Estonia; Estonian Academy of Sciences, Kohtu 6, 10130 Tallinn, Estonia. Electronic address: mart.ustav@ut.ee.

PMID: 29179037
DOI: 10.1016/j.virol.2017.11.013

Abstract

Nuclear myosin 1c (NM1) associates with RNA polymerases and is a partner in the chromatin remodeling complex B-WICH. This complex, which also contains WSTF and SNF2h proteins, is involved in transcriptional regulation. We report herein that papillomavirus protein E2 binds to NM1 and co-precipitates with the WSTF and SNF2h proteins. Our data suggest that E2 associates with the cellular B-WICH complex through binding to NM1. E2 and NM1 associate via their N-terminal domains and this interaction is ATP dependent. The cellular multifunctional protein Brd4 and beta-actin are also present in the NM1-E2 complex. NM1 downregulation by siRNA increases the replication of the BPV1 and HPV5 genomes but does not affect HPV18 genome replication. These results suggest that the B-WICH complex may play a role in the papillomavirus life cycle through NM1 and E2 protein interaction.

Keywords: E2 protein; Nuclear myosin I; Papillomavirus; Viral replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism
Betapapillomavirus / genetics
Betapapillomavirus / metabolism*
Bovine papillomavirus 1 / genetics
Bovine papillomavirus 1 / metabolism
Cell Cycle Proteins
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Host-Pathogen Interactions
Human papillomavirus 18 / chemistry
Human papillomavirus 18 / genetics
Human papillomavirus 18 / metabolism*
Humans
Myosin Type I / chemistry
Myosin Type I / genetics
Myosin Type I / metabolism*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Oncogene Proteins, Viral / genetics
Oncogene Proteins, Viral / metabolism*
Papillomavirus Infections / genetics
Papillomavirus Infections / metabolism*
Papillomavirus Infections / virology
Protein Binding
Protein Domains
Transcription Factors / genetics
Transcription Factors / metabolism
Viral Proteins / genetics
Viral Proteins / metabolism
Virus Replication*

Substances

BAZ1B protein, human
BRD4 protein, human
Cell Cycle Proteins
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
E2 protein, Bovine papillomavirus
Nuclear Proteins
Oncogene Proteins, Viral
Transcription Factors
Viral Proteins
oncogene protein E2, Human papillomavirus type 1
Adenosine Triphosphatases
Myosin Type I
SMARCA5 protein, human
MYO1C protein, human