[Knock-down of KHDRBS3 gene inhibits proliferation of human ovarian cancer CAOV-3 cells]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Aug;33(8):1062-1066.
[Article in Chinese]

Abstract

Objective To investigate the effect of KH RNA binding domain-containing, signal transduction-associated 3 (KHDRBS3) gene on the proliferation of human ovarian cancer CAOV-3 cells in vitro. Methods The logarithmic growth phase cells were divided into non-transfection group, unrelated small interfering RNA (siRNA) sequence transfection group and KHDRBS3-siRNA transfection group. Reverse transcription PCR and Western blotting were used to verify the result of KHDRBS3 gene silencing. The proliferation of CAOV-3 cells in different groups was compared by MTT assay. The cell cycle distribution of CAOV-3 cells in different treatment groups was detected by flow cytometry. Results KHDRBS3-siRNA transfection significantly decreased the mRNA and protein levels of KHDRBS3 gene in CAOV-3 cells. After the knock-down of KHDRBS3 gene in CAOV-3 cells, the proliferation of the cells markedly decreased, the percentage of G0/G1 phase cells increased, and the percentage of S phase cells decreased, while the percentage of G2/M phase cells did not change significantly. Conclusion Down-regulating the KHDRBS3 gene can cause G0/G1 phase arrest and inhibit cell proliferation in CAOV-3 cells.

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / physiology
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology
  • Female
  • G1 Phase Cell Cycle Checkpoints
  • Humans
  • Ovarian Neoplasms / pathology*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / physiology*

Substances

  • Adaptor Proteins, Signal Transducing
  • DNA-Binding Proteins
  • KHDRBS1 protein, human
  • KHDRBS3 protein, human
  • RNA-Binding Proteins