Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis

Cell Rep. 2017 Aug 15;20(7):1597-1608. doi: 10.1016/j.celrep.2017.07.067.

Abstract

We analyzed chromatin dynamics and transcriptional activity of human embryonic stem cell (hESC)-derived cardiac progenitor cells (CPCs) and KDR+/CD34+ endothelial cells generated from different mesodermal origins. Using an unbiased algorithm to hierarchically rank genes modulated at the level of chromatin and transcription, we identified candidate regulators of mesodermal lineage determination. HOPX, a non-DNA-binding homeodomain protein, was identified as a candidate regulator of blood-forming endothelial cells. Using HOPX reporter and knockout hESCs, we show that HOPX regulates blood formation. Loss of HOPX does not impact endothelial fate specification but markedly reduces primitive hematopoiesis, acting at least in part through failure to suppress Wnt/β-catenin signaling. Thus, chromatin state analysis permits identification of regulators of mesodermal specification, including a conserved role for HOPX in governing primitive hematopoiesis.

Keywords: Wnt signaling; cardiac; cardiovascular; chromatin dynamics; differentiation; epigenetics; genome engineering; hematopoiesis; human pluripotent stem cell.

MeSH terms

  • Algorithms
  • CRISPR-Cas Systems
  • Cell Differentiation
  • Cell Lineage / genetics
  • Chromatin / chemistry
  • Chromatin / metabolism*
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Genes, Reporter
  • Hematopoiesis / genetics*
  • Homeodomain Proteins / genetics*
  • Human Embryonic Stem Cells / cytology
  • Human Embryonic Stem Cells / metabolism*
  • Humans
  • Mesoderm / cytology
  • Mesoderm / growth & development
  • Mesoderm / metabolism*
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / metabolism
  • Signal Transduction
  • T-Cell Acute Lymphocytic Leukemia Protein 1 / genetics*
  • T-Cell Acute Lymphocytic Leukemia Protein 1 / metabolism
  • Transcription, Genetic*
  • Tumor Suppressor Proteins / deficiency
  • Tumor Suppressor Proteins / genetics*
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • CTNNB1 protein, human
  • Chromatin
  • Fluorescent Dyes
  • HOPX protein, human
  • Homeodomain Proteins
  • T-Cell Acute Lymphocytic Leukemia Protein 1
  • Tumor Suppressor Proteins
  • beta Catenin
  • TAL1 protein, human