Identification of STS-1 as a novel ShcA-binding protein

Talitha van der Meulen; Spencer Swarts; Wolfgang Fischer; Peter van der Geer

doi:10.1016/j.bbrc.2017.07.024

Identification of STS-1 as a novel ShcA-binding protein

Biochem Biophys Res Commun. 2017 Sep 2;490(4):1334-1339. doi: 10.1016/j.bbrc.2017.07.024. Epub 2017 Jul 6.

Authors

Talitha van der Meulen¹, Spencer Swarts¹, Wolfgang Fischer², Peter van der Geer³

Affiliations

¹ Department of Chemistry and Biochemistry, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-1030, USA.
² Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, 10010 North Torrey Pines Rd, La Jolla, CA 92037, USA.
³ Department of Chemistry and Biochemistry, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-1030, USA. Electronic address: pvanderg@mail.sdsu.edu.

PMID: 28690151
DOI: 10.1016/j.bbrc.2017.07.024

Abstract

ShcA is a cytoplasmic signaling protein that supports signal transduction by receptor protein-tyrosine kinases by providing auxiliary tyrosine phosphorylation sites that engage additional signaling proteins. The principal binding partner for tyrosine phosphorylation sites on ShcA is Grb2. In the current study, we have used phosphotyrosine-containing peptides to isolate and identify STS-1 as a novel ShcA-binding protein. Our results further show that the interaction between STS-1 and ShcA is regulated in response to EGF receptor activation.

Keywords: Phosphopeptide affinity purification; Phosphotyrosine; Protein-tyrosine kinase; Signal transduction.

MeSH terms

A549 Cells
Amino Acid Sequence
Animals
Binding Sites
COS Cells
Chlorocebus aethiops
Epidermal Growth Factor / genetics*
Epidermal Growth Factor / metabolism
Gene Expression
Humans
Peptides / chemical synthesis
Peptides / genetics*
Peptides / metabolism
Phosphoproteins / genetics*
Phosphoproteins / metabolism
Phosphorylation
Phosphotyrosine / metabolism*
Protein Binding
Protein Tyrosine Phosphatases / genetics*
Protein Tyrosine Phosphatases / metabolism
Sequence Alignment
Signal Transduction
Src Homology 2 Domain-Containing, Transforming Protein 1 / genetics*
Src Homology 2 Domain-Containing, Transforming Protein 1 / metabolism

Substances

Peptides
Phosphoproteins
SHC1 protein, human
Src Homology 2 Domain-Containing, Transforming Protein 1
Phosphotyrosine
Epidermal Growth Factor
Protein Tyrosine Phosphatases
UBASH3B protein, human