MicroRNA-214 regulates immunity-related genes in bovine mammary epithelial cells by targeting NFATc3 and TRAF3

Mol Cell Probes. 2017 Oct:35:27-33. doi: 10.1016/j.mcp.2017.06.002. Epub 2017 Jun 13.

Abstract

In human, microRNA-214 (miR-214) plays crucial roles in mechanisms of immunity. However, the potential importance of miR-214 in immune mechanisms in dairy cows has not been investigated. In this study, we assessed potential immunity-related functions of miR-214 in human 293A cells and in bovine mammary epithelial cells (BMECs). We found that NFATc3 and TRAF3 could be targeted by miR-214 in both 293A cells and BMECs. We also found that miR-214 indirectly inhibited the expression of MAP3K14, TBK1 and inflammatory cytokines IL-6 and IL-1β. Taken together, our data revealed miR-214 regulated immunity-related genes by targeting NFATc3 and TRAF3, which provides insight into the molecular basis of immunity.

Keywords: BMECs; Immunity; NFATc3; TRAF3; miR-214.

MeSH terms

  • Animals
  • Cattle
  • Cell Line
  • Epithelial Cells / metabolism*
  • Female
  • Humans
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Mammary Glands, Animal / cytology*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • NFATC Transcription Factors / genetics
  • NFATC Transcription Factors / metabolism*
  • Protein Serine-Threonine Kinases / metabolism
  • TNF Receptor-Associated Factor 3 / genetics
  • TNF Receptor-Associated Factor 3 / metabolism*

Substances

  • Interleukin-1beta
  • Interleukin-6
  • MIRN214 microRNA, human
  • MicroRNAs
  • NFATC Transcription Factors
  • TNF Receptor-Associated Factor 3
  • transcription factor NF-AT c3
  • Protein Serine-Threonine Kinases
  • TBK1 protein, human