Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish

Ya Zhu; Guanyong Su; Dandong Yang; Yongkang Zhang; Liqin Yu; Yufei Li; John P Giesy; Robert J Letcher; Chunsheng Liu

doi:10.1016/j.envpol.2017.06.024

Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish

Environ Pollut. 2017 Oct:229:470-478. doi: 10.1016/j.envpol.2017.06.024. Epub 2017 Jun 15.

Authors

Ya Zhu¹, Guanyong Su², Dandong Yang¹, Yongkang Zhang¹, Liqin Yu¹, Yufei Li³, John P Giesy⁴, Robert J Letcher⁵, Chunsheng Liu⁶

Affiliations

¹ College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
² Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
³ China Rural Technology Development Centre, Ministry of Science and Technology of PR China, Beijing 100045, China.
⁴ State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210089, China; Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada; Department of Zoology and Centre for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, United States.
⁵ Department of Chemistry, Carleton University, Ottawa, Ontario K1S 5B6, Canada.
⁶ College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Collbaorative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan, Changde 415000, China. Electronic address: liuchunshengidid@126.com.

PMID: 28624628
DOI: 10.1016/j.envpol.2017.06.024

Abstract

Growth curves were used to determine sensitive exposure windows for evaluation of developmental toxicity of chemicals to zebrafish. Dose- and time-dependent effects on body mass, body length and expression of genes involved in the growth hormone/insulin-like growth factor (GH/IGF) axis and the hypothalamic-pituitary-thyroid (HPT) axis were examined after exposure to environmentally relevant concentrations of tris(1,3-dichloro-2-propyl) phosphate (TDCIPP). Based on growth curves, zebrafish grew most rapidly between 60 and 90 days post fertilization (dpf). Exposure to environmentally relevant concentrations of TDCIPP significantly decreased body mass and body length and down-regulated expression of several genes involved in the GH/IGF axis of female zebrafish, but no such effects were observed in male zebrafish. Exposure to TDCIPP did not change concentrations of thyroid hormones or expression of genes along the HPT axis in female and male zebrafish. These results suggest that growth stages of zebrafish between 60 and 90 dpf might be most appropriate for evaluation of developmental toxicity of chemicals, and down-regulation of genes involved in the GH/IGF axis, but not the HPT axis, might be responsible for the observed growth inhibition in females exposed to TDCIPP.

Keywords: Developmental toxicity; Growth hormone/insulin-like growth factor (GH/IGF) axis; Hypothalamic-pituitary-thyroid (HPT) axis; Organophosphate flame retardant.

MeSH terms

Animals
Down-Regulation
Female
Gene Expression / drug effects*
Male
Organophosphates
Organophosphorus Compounds / toxicity*
Thyroid Hormones / metabolism
Water Pollutants, Chemical / toxicity*
Zebrafish / metabolism
Zebrafish / physiology*

Substances

Organophosphates
Organophosphorus Compounds
Thyroid Hormones
Water Pollutants, Chemical
tris(1,3-dichloro-2-propyl)phosphate