Abstract
The Src-family tyrosine kinase Lck is an enzyme associated with the CD4 and CD8 co-receptors and promoting signaling through the T cell receptor (TCR) complex. The levels of Lck expression and activity change during the development and differentiation of T cells. Here we show that Lck expression is higher in Th1 cells as compared to Th2 cells. Ectopic overexpression of Lck in Th2 cells results in increased expression of CD4 co-receptor and enhanced S73 phosphorylation of transcription factor c-Jun. Our findings indicate that TCR-mediated signaling in Th2 cells may be directly attenuated by Lck protein expression level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD4-Positive T-Lymphocytes / metabolism*
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Cells, Cultured
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Gene Expression Profiling
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HEK293 Cells
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Humans
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / genetics
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / metabolism*
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Mice, Inbred C57BL
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Phosphorylation
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Proto-Oncogene Proteins c-jun / metabolism*
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Receptors, Antigen, T-Cell / metabolism*
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Serine / metabolism
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Signal Transduction
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Th1 Cells / metabolism
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Th2 Cells / metabolism*
Substances
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Proto-Oncogene Proteins c-jun
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Receptors, Antigen, T-Cell
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Serine
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)