DDX53 Regulates Cancer Stem Cell-Like Properties by Binding to SOX-2

Mol Cells. 2017 May 31;40(5):322-330. doi: 10.14348/molcells.2017.0001. Epub 2017 May 2.

Abstract

This study investigated the role of cancer/testis antigen DDX53 in regulating cancer stem cell-like properties. DDX53 shows co-expression with CD133, a marker for cancer stem cells. DDX53 directly regulates the SOX-2 expression in anticancer drug-resistant Malme3MR cells. DDX53 and miR-200b were found to be involved in the regulation of tumor spheroid forming potential of Malme3M and Malme3MR cells. Furthermore, the self-renewal activity and the tumorigenic potential of Malme3MR-CD133 (+) cells were also regulated by DDX53. A miR-200b inhibitor induced the direct regulation of SOX-2 by DDX53 We therefore, conclude that DDX53 may serve as an immunotherapeutic target for regulating cancer stem-like properties of melanomas.

Keywords: DDX53; SOX-2; anti-cancer drug-resistance; cancer stem cell-like properties.

MeSH terms

  • AC133 Antigen / metabolism
  • Animals
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Cell Line, Tumor
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism*
  • Drug Resistance, Neoplasm / drug effects
  • Drug Resistance, Neoplasm / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Melanoma / metabolism
  • Melanoma / pathology
  • Mice, Inbred BALB C
  • MicroRNAs / genetics
  • Neoplastic Stem Cells / metabolism
  • Neoplastic Stem Cells / pathology*
  • Paclitaxel / pharmacology
  • SOXB1 Transcription Factors / genetics
  • SOXB1 Transcription Factors / metabolism*
  • Xenograft Model Antitumor Assays

Substances

  • AC133 Antigen
  • Antineoplastic Agents, Phytogenic
  • MIRN200 microRNA, human
  • MicroRNAs
  • PROM1 protein, human
  • SOX2 protein, human
  • SOXB1 Transcription Factors
  • DDX53 protein, human
  • DEAD-box RNA Helicases
  • Paclitaxel