MiR-9-3p augments apoptosis induced by H2O2 through down regulation of Herpud1 in glioma

PLoS One. 2017 Apr 21;12(4):e0174839. doi: 10.1371/journal.pone.0174839. eCollection 2017.

Abstract

MicroRNAs are short, single-stranded non-coding RNA molecules that function as regulators of tumor progression in various cancers, including glioma. The present study sought to investigate the biological functions of miR-9-3p in glioma progression. The results of a microRNA microarray indicated that microRNA-9-3p (miR-9-3p, miR-9*) is down-regulated in high-grade (grades III and IV) gliomas compared with non-tumor tissues. These results were confirmed with real-time PCR. The miR-9-3p expression level was associated with age and tumor grade. Herpud1 was regulated by miR-9-3p in glioma cells and tissues and was identified as a miR-9-3p target with luciferase reporter assays. Glioma cells transfected with miR-9-3p mimics or HERPUD1-RNAi had more apoptotic cells than them in control after induced by H2O2. Our results indicated that low expression of miR-9-3p results in a high level of Herpud1, which may protect against apoptosis in glioma.

MeSH terms

  • 3' Untranslated Regions
  • Adult
  • Apoptosis / drug effects*
  • Apoptosis / genetics
  • Blotting, Western
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology*
  • Down-Regulation / drug effects*
  • Female
  • Gene Expression Profiling
  • Glioma / metabolism
  • Glioma / pathology*
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Male
  • Membrane Proteins / metabolism*
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Middle Aged
  • Real-Time Polymerase Chain Reaction

Substances

  • 3' Untranslated Regions
  • HERPUD1 protein, human
  • MIRN92 microRNA, human
  • Membrane Proteins
  • MicroRNAs
  • Hydrogen Peroxide

Grants and funding

Our study was supported by Autonomous Tumor Innovation Team (No Funding Number) and Laboratory of Ministry Funding (X201005 and X201002) to XS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.