DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair

Nucleic Acids Res. 2017 Jun 2;45(10):5901-5912. doi: 10.1093/nar/gkx261.

Abstract

Repair of damaged DNA relies on the recruitment of DNA repair factors in a well orchestrated manner. As a prerequisite, the chromatin needs to be decondensed by chromatin remodelers to allow for binding of repair factors and for DNA repair to occur. Recent studies have implicated members of the SWI/SNF and INO80 families as well as PARP1 in nucleotide excision repair (NER). In this study, we report that the endonuclease DICER is implicated in chromatin decondensation during NER. In response to UV irradiation, DICER is recruited to chromatin in a ZRF1-mediated manner. The H2A-ubiquitin binding protein ZRF1 and DICER together impact on the chromatin conformation via PARP1. Moreover, DICER-mediated chromatin decondensation is independent of its catalytic activity. Taken together, we describe a novel function of DICER at chromatin and its interaction with the ubiquitin signalling cascade during GG-NER.

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans / radiation effects
  • Cell Line
  • Cell Line, Tumor
  • Chromatin / chemistry*
  • Chromatin / metabolism
  • Chromatin Assembly and Disassembly
  • DEAD-box RNA Helicases / genetics*
  • DEAD-box RNA Helicases / metabolism
  • DNA Damage
  • DNA Repair*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibroblasts / radiation effects
  • HEK293 Cells
  • Histones / genetics
  • Histones / metabolism
  • Humans
  • Molecular Chaperones
  • Oncogene Proteins / genetics*
  • Oncogene Proteins / metabolism
  • Osteoblasts / cytology
  • Osteoblasts / metabolism
  • Osteoblasts / radiation effects
  • Poly (ADP-Ribose) Polymerase-1 / genetics
  • Poly (ADP-Ribose) Polymerase-1 / metabolism
  • RNA-Binding Proteins
  • Ribonuclease III / genetics*
  • Ribonuclease III / metabolism
  • Ubiquitin / genetics
  • Ubiquitin / metabolism
  • Ultraviolet Rays

Substances

  • Chromatin
  • DNA-Binding Proteins
  • DNAJC2 protein, human
  • Histones
  • Molecular Chaperones
  • Oncogene Proteins
  • RNA-Binding Proteins
  • Ubiquitin
  • PARP1 protein, human
  • Poly (ADP-Ribose) Polymerase-1
  • DICER1 protein, human
  • Ribonuclease III
  • DEAD-box RNA Helicases