miR-1193 Suppresses the Proliferation and Invasion of Human T-Cell Leukemia Cells Through Directly Targeting the Transmembrane 9 Superfamily 3 (TM9SF3)

Oncol Res. 2017 Nov 2;25(9):1643-1651. doi: 10.3727/096504017X14908284471361. Epub 2017 Mar 30.

Abstract

miRNAs have been involved in various types of cancer, including T-cell leukemia. In this study, the role of miR-1193 in the proliferation and invasion of T-cell leukemia cells was explored. First, we found that miR-1193 was sharply downregulated in T-cell leukemia cells when compared with normal T cells. miR-1193 markedly decreased the proliferation and invasion in Jurkat human T-cell leukemia cells. Transmembrane 9 superfamily 3 (TM9SF3) was then predicted to be a potential target gene of miR-1193, the levels of which displayed a strongly negative correlation with miR-1193 levels in T-cell leukemia patients. We confirmed that TM9SF3 was a target gene of miR-1193 by luciferase reporter gene assay. Finally, gene overexpression and knockdown experiments in Jurkat cells revealed that TM9SF3 positively regulated cell proliferation and invasion.

MeSH terms

  • Adult
  • Carrier Proteins
  • Cell Line, Tumor
  • Cell Proliferation / physiology
  • Down-Regulation
  • Humans
  • Jurkat Cells
  • Leukemia / genetics*
  • Leukemia / metabolism
  • Leukemia / pathology
  • Leukemia, T-Cell / genetics*
  • Leukemia, T-Cell / metabolism
  • Leukemia, T-Cell / pathology
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Transfection

Substances

  • Carrier Proteins
  • Membrane Proteins
  • MicroRNAs
  • TM9SF3 protein, human