Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia CEM cells

Exp Oncol. 2017 Mar;39(1):36-41.

Abstract

The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells.

Materials and methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein G-Sepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis.

Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm.

Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens.

MeSH terms

  • Antibodies, Anti-Idiotypic / isolation & purification
  • Antibodies, Anti-Idiotypic / metabolism
  • Antibodies, Anti-Idiotypic / pharmacology*
  • Antibody Affinity / immunology
  • Antibody Specificity / immunology
  • Blotting, Western
  • Cell Cycle / drug effects*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • Chromatography, Affinity
  • Chromatography, Gel
  • Cross Reactions / immunology
  • Cytoplasm / metabolism
  • Histones / immunology*
  • Humans
  • Leukemia, T-Cell / immunology
  • Leukemia, T-Cell / metabolism
  • Leukemia, T-Cell / pathology
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology

Substances

  • Antibodies, Anti-Idiotypic
  • Histones
  • anti-IgG