Polymorphisms of Platelet Glycoprotein Receptors and Cell Adhesion Molecules in Fetuses with Fetal Growth Restriction and Their Mothers As Detected with Pyrosequencing

Maria Simou; Evaggelia Kouskouni; Nikolaos Vitoratos; Emmanuel Economou; George Creatsas

doi:10.21873/invivo.11052

Polymorphisms of Platelet Glycoprotein Receptors and Cell Adhesion Molecules in Fetuses with Fetal Growth Restriction and Their Mothers As Detected with Pyrosequencing

In Vivo. 2017 Mar-Apr;31(2):243-249. doi: 10.21873/invivo.11052.

Authors

Maria Simou¹, Evaggelia Kouskouni², Nikolaos Vitoratos³, Emmanuel Economou², George Creatsas³

Affiliations

¹ Second Department of Obstetrics and Gynecology, Medical School, Aretaieio Hospital, National and Kapodistrian University of Athens, Athens, Greece maria.simou@ymail.com.
² Laboratory of Therapeutic Individualization, Second Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, Medical School, Aretaieio Hospital, Athens, Greece.
³ Second Department of Obstetrics and Gynecology, Medical School, Aretaieio Hospital, National and Kapodistrian University of Athens, Athens, Greece.

Abstract

Background: Vascular thrombotic tendency may lead to fetal growth restriction (FGR). Altered platelet function and genetic heterogeneity may play a role in this procedure. We investigated whether maternal or fetal genotypic frequencies of genes polymorphisms for certain platelet receptor and cell adhesion molecules are altered in FGR.

Materials and methods: We compared the maternal and fetal genotypic frequencies of single nucleotide polymorphisms (SNPs) in four genes coding for platelet receptors and cell adhesion molecules [integrin alpha subunit 2 (ITGA2)C807T, integrin subunit beta 3(ITGB3) T1565C, platelet cell adhesion protein 1 (PECAM1) CTG-GTG and selectin P(SELP)A/C]. A total of 32 fetuses with fetal growth restriction and their mothers were matched with 18 normal controls. Using maternal venous blood and umbilical cord blood samples, nucleotide sequences were determined from pyrograms. Genotypic frequencies were calculated and analyzed using appropriate tests and logistic regression.

Results: There was no statistical difference in the proportion of heterozygotes or homozygotes for any of the genotypic frequencies between FGR and control groups in mothers or fetuses.

Conclusion: Our study demonstrated no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with FGR.

Keywords: FGR; SNPs; platelet glycoproteins; pyrosequencing.

MeSH terms

Adult
Cell Adhesion Molecules / genetics*
Female
Fetal Growth Retardation / genetics*
Fetus / metabolism
Gene Frequency
Genotype
Humans
Integrin alpha2 / genetics
Integrin beta3 / genetics
Logistic Models
Mothers
P-Selectin / genetics
Platelet Endothelial Cell Adhesion Molecule-1 / genetics
Platelet Membrane Glycoproteins / genetics*
Polymorphism, Single Nucleotide*
Prospective Studies
Sequence Analysis, DNA / methods*

Substances

Cell Adhesion Molecules
ITGA2B protein, human
ITGB3 protein, human
Integrin alpha2
Integrin beta3
P-Selectin
Platelet Endothelial Cell Adhesion Molecule-1
Platelet Membrane Glycoproteins
SELP protein, human