The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation

Nat Cell Biol. 2017 Apr;19(4):384-390. doi: 10.1038/ncb3486. Epub 2017 Mar 6.

Abstract

Microtubules tether centrosomes together during interphase. How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end-directed kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome separation during interphase. We show that premature centrosome separation leads to microtubule-dependent nuclear translocation, culminating in eccentric nuclear positioning that disrupts the cortical spindle positioning machinery. The activity of Kif25 during interphase is required to maintain a centred nucleus to ensure the spindle is stably oriented at the onset of mitosis.

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Centrosome / metabolism*
  • Green Fluorescent Proteins / metabolism
  • HCT116 Cells
  • HeLa Cells
  • Humans
  • Kinesins / chemistry*
  • Kinesins / metabolism*
  • LLC-PK1 Cells
  • Mitosis*
  • Protein Multimerization*
  • Spindle Apparatus / metabolism*
  • Swine

Substances

  • KIF25 protein, human
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Kinesins