LY6/PLAUR domain containing 3 has a role in the maintenance of colorectal cancer stem-like cells

Liming Wang; Yoshihiko Hirohashi; Tadashi Ogawa; Min Shen; Rui Takeda; Aiko Murai; Eri Yamamoto; Terufumi Kubo; Munehide Nakatsugawa; Takayuki Kanaseki; Tomohide Tsukahara; Toshihiko Nishidate; Kenji Okita; Goro Kutomi; Noriyuki Sato; Ichiro Takemasa; Toshihiko Torigoe

doi:10.1016/j.bbrc.2017.02.112

LY6/PLAUR domain containing 3 has a role in the maintenance of colorectal cancer stem-like cells

Biochem Biophys Res Commun. 2017 Apr 29;486(2):232-238. doi: 10.1016/j.bbrc.2017.02.112. Epub 2017 Feb 24.

Authors

Liming Wang¹, Yoshihiko Hirohashi², Tadashi Ogawa³, Min Shen⁴, Rui Takeda⁴, Aiko Murai⁴, Eri Yamamoto⁴, Terufumi Kubo⁴, Munehide Nakatsugawa⁴, Takayuki Kanaseki⁴, Tomohide Tsukahara⁴, Toshihiko Nishidate⁵, Kenji Okita⁵, Goro Kutomi⁵, Noriyuki Sato⁴, Ichiro Takemasa⁵, Toshihiko Torigoe⁶

Affiliations

¹ Department of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan; Department of Surgery, Sapporo Higashi Tokusyukai Hospital, North-33 East-14, Higashi-Ku, Sapporo 065-0033, Japan.
² Department of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan. Electronic address: hirohash@sapmed.ac.jp.
³ Department of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan; Department of Surgery, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan.
⁴ Department of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan.
⁵ Department of Surgery, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan.
⁶ Department of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan. Electronic address: torigoe@sapmed.ac.jp.

PMID: 28238780
DOI: 10.1016/j.bbrc.2017.02.112

Abstract

Colorectal carcinoma (CRC) is one of the most frequently diagnosed cancers and the leading cause of cancer-related death for both men and women. Recent studies have revealed that a small sub-population of cancer cells, termed cancer stem-like cells (CSCs)/cancer-initiating cells (CICs), are endowed with tumor-initiating ability, self-renewal ability and differentiation ability. CSCs/CICs are resistant to current therapies including chemotherapy and radiotherapy. Thus, CSCs/CICs are responsible for recurrence and metastasis, and eradication of CSCs/CICs is essential to cure cancer. In this study, we isolated CR-CSCs/CICs as sphere-cultured cells and found that a product derived from LY6/PLAUR domain containing 3 (LYPD3) is preferentially expressed in CSCs/CICs. Gene overexpression and gene knockdown experiments revealed that LYPD3 has a role in the maintenance of CR-CSCs/CICs. The findings provide a novel molecular insight into CR-CSCs/CICs.

Keywords: Cancer stem cell; Colorectal cancer; LYPD3; Sphere formation.

MeSH terms

Aldehyde Dehydrogenase / genetics
Aldehyde Dehydrogenase / metabolism
Aldehyde Dehydrogenase 1 Family
Cell Adhesion Molecules / antagonists & inhibitors
Cell Adhesion Molecules / genetics*
Cell Adhesion Molecules / metabolism
Cell Line, Tumor
Cell Proliferation
GPI-Linked Proteins / antagonists & inhibitors
GPI-Linked Proteins / genetics
GPI-Linked Proteins / metabolism
Gene Expression Regulation, Neoplastic*
HCT116 Cells
HT29 Cells
Humans
Nanog Homeobox Protein / genetics
Nanog Homeobox Protein / metabolism
Neoplastic Stem Cells / metabolism*
Neoplastic Stem Cells / pathology
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Retinal Dehydrogenase
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism
Signal Transduction
Spheroids, Cellular / metabolism*
Spheroids, Cellular / pathology

Substances

Cell Adhesion Molecules
GPI-Linked Proteins
LYPD3 protein, human
NANOG protein, human
Nanog Homeobox Protein
Octamer Transcription Factor-3
POU5F1 protein, human
RNA, Small Interfering
SOX2 protein, human
SOXB1 Transcription Factors
Aldehyde Dehydrogenase 1 Family
Aldehyde Dehydrogenase
ALDH1A1 protein, human
Retinal Dehydrogenase