MicroRNA-155 targets MAP3K10 and regulates osteosarcoma cell growth

Pathol Res Pract. 2017 Apr;213(4):389-393. doi: 10.1016/j.prp.2016.12.028. Epub 2017 Feb 1.

Abstract

Osteosarcoma is the most common type of bone cancer prevalent in young adults. Recent studies suggested that aberrant microRNA expression plays an essential role in osteosarcoma pathogenesis. In this study, we found miR-155 up-regulation in different osteosarcoma cell lines U2OS, Saos-2 and MG-63. Through bioinformatic prediction and analysis, we identified its target MAP3K10 that involved in cell proliferation and apoptosis. This work demonstrates novel interaction between microRNA, intercellular MAPK signaling and apoptosis in osteosarcoma, which will provide targets for therapeutic development.

Keywords: Cell lines; MAP4K10; MicroRNA-155; Osteosarcoma.

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic / physiology*
  • Gene Knockdown Techniques
  • Heterografts
  • Humans
  • MAP Kinase Kinase Kinases / biosynthesis*
  • Mice
  • Mice, Nude
  • MicroRNAs / genetics*
  • Osteosarcoma / pathology*
  • Real-Time Polymerase Chain Reaction

Substances

  • MIRN155 microRNA, human
  • MicroRNAs
  • MAP Kinase Kinase Kinases
  • MAP3K10 protein, human