"HLA-G 3'UTR gene polymorphisms and rheumatic heart disease: a familial study among South Indian population"

Pediatr Rheumatol Online J. 2017 Feb 1;15(1):10. doi: 10.1186/s12969-017-0140-x.

Abstract

Background: Rheumatic heart disease (RHD) is an autoimmune disease where cross reactive CD4+ T cells are involved in the pathogenesis of valvular damage. Human Leukocyte Antigen-G (HLA-G), an immunosuppressive molecule playing a crucial role in the inhibition of T cell response is associated with the pathogenesis of various autoimmune and inflammatory diseases. Genetic polymorphisms within the 3'untranslated region (UTR) of HLA-G influences its expression and thus disease pathogenesis. Hence, the present study aims to unravel the association of 14 bp Ins/Del (rs66554220) and +3142 C/G (rs1063320) polymorphisms in 3' UTR of HLA-G with RHD.

Methods: This familial study consists of 99 RHD families (99 RHD patients, 140 parents and 126 healthy siblings). The 14 bp Ins/Del and +3142 C/G polymorphisms were evaluated by PCR using sequence specific primers and its transmission disequilibrium (TD) was tested by TD test in 70 trio families.

Results: The frequency of +3142 C/C genotype was high in patients with combined valvular lesions (CVL) (OR = 5.88; pc = 0.012) and pooled RHD patients (P: OR = 2.76; p = 0.043; pc = 0.076) when compared to healthy siblings. Under the additive (OR = 5.50; pc = 0.026) and recessive genetic model (OR = 5.88; pc = 0.012), the +3142 C/C genotype was significantly associated with CVL in patients.

Conclusion: The results suggest that the +3142 C/C genotype may be associated with minor risk for the development of RHD and is more likely to influence the severity of the disease.

Keywords: Autoimmunity; Human leukocyte antigen G; Polymorphism; Rheumatic fever; Rheumatic heart disease.

MeSH terms

  • 3' Untranslated Regions / genetics*
  • Child
  • Female
  • Genotype
  • HLA-G Antigens / genetics*
  • Humans
  • India / ethnology
  • Linkage Disequilibrium
  • Male
  • Pilot Projects
  • Polymorphism, Genetic / genetics*
  • Retrospective Studies
  • Rheumatic Heart Disease / genetics*

Substances

  • 3' Untranslated Regions
  • HLA-G Antigens