Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells

Miao Li; Qiang Dong; Bing Zhu

doi:10.1016/j.jmb.2017.01.016

Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells

J Mol Biol. 2017 Jun 30;429(13):2042-2045. doi: 10.1016/j.jmb.2017.01.016. Epub 2017 Jan 27.

Authors

Miao Li¹, Qiang Dong², Bing Zhu³

Affiliations

¹ Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, 100081, China; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; National Institute of Biological Sciences, Beijing 102206, China.
² National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
³ National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zhubing@ibp.ac.cn.

PMID: 28137420
DOI: 10.1016/j.jmb.2017.01.016

Abstract

Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro.

Keywords: Aurora kinase B; H3.3S31ph; histone phosphorylation; siRNA screen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aurora Kinase B / metabolism*
Gene Expression Regulation*
Gene Silencing
Genetic Testing
Histones / metabolism*
Humans
Mitosis*
Phosphorylation
Protein Processing, Post-Translational*
Serine / metabolism*

Substances

Histones
Serine
AURKB protein, human
Aurora Kinase B