The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival

Invest Ophthalmol Vis Sci. 2017 Jan 1;58(1):448-460. doi: 10.1167/iovs.16-20326.

Abstract

Purpose: Joubert syndrome (JBTS) is an autosomal recessive ciliopathy with considerable phenotypic variability. In addition to central nervous system abnormalities, a subset of JBTS patients exhibit retinal dystrophy and/or kidney disease. Mutations in the AHI1 gene are causative for approximately 10% of all JBTS cases. The purpose of this study was to generate ahi1 mutant alleles in zebrafish and to characterize the retinal phenotypes.

Methods: Zebrafish ahi1 mutants were generated using transcription activator-like effector nucleases (TALENs). Expression analysis was performed by whole-mount in situ hybridization. Anatomic and molecular characterization of photoreceptors was investigated by histology, electron microscopy, and immunohistochemistry. The optokinetic response (OKR) behavior assay was used to assess visual function. Kidney cilia were evaluated by whole-mount immunostaining.

Results: The ahi1lri46 mutation in zebrafish resulted in shorter cone outer segments but did not affect visual behavior at 5 days after fertilization (dpf). No defects in rod morphology or rhodopsin localization were observed at 5 dpf. By 5 months of age, cone degeneration and rhodopsin mislocalization in rod photoreceptors was observed. The connecting cilium formed normally and Cc2d2a and Cep290 localized properly. Distal pronephric duct cilia were absent in mutant fish; however, only 9% of ahi1 mutants had kidney cysts by 5 dpf, suggesting that the pronephros remained largely functional.

Conclusions: The results indicate that Ahi1 is required for photoreceptor disc morphogenesis and outer segment maintenance in zebrafish.

MeSH terms

  • Animals
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Survival
  • Cilia / metabolism
  • Cilia / ultrastructure*
  • Ciliopathies / genetics*
  • Ciliopathies / metabolism
  • Ciliopathies / pathology
  • DNA Mutational Analysis
  • Genotype
  • Immunohistochemistry
  • In Situ Hybridization
  • Microscopy, Electron, Transmission
  • Morphogenesis*
  • Mutation*
  • Proto-Oncogene Proteins
  • Retinal Photoreceptor Cell Outer Segment / metabolism
  • Retinal Photoreceptor Cell Outer Segment / ultrastructure*
  • Zebrafish
  • Zebrafish Proteins / genetics*
  • Zebrafish Proteins / metabolism

Substances

  • Ahi1 protein, zebrafish
  • Carrier Proteins
  • Proto-Oncogene Proteins
  • Zebrafish Proteins