Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop

Biochem Biophys Res Commun. 2017 Mar 11;484(3):514-521. doi: 10.1016/j.bbrc.2017.01.094. Epub 2017 Jan 20.

Abstract

The long noncoding RNA (lncRNA) ATB is an important regulator in human tumors. Here, we aimed to investigate the potential molecular mechanisms of lnc-ATB in gastric cancer (GC) tumorigenesis. RT-qPCR analysis was used to detect lnc-ATB expression level in 20 pairs of gastric cancer tissues and adjacent normal gastric mucosa tissues (ANTs). Moreover, the biological role of lnc-ATB was determined in vitro. We found that lnc-ATB was significantly upregulated in GC tissues compared to lnc-ATB expression in ANTs. These high lnc-ATB expression levels predicted poor prognosis in GC patients. Low levels of lnc-ATB inhibited GC cell proliferation and cell cycle arrest in vitro. Lnc-ATB was found to directly bind miR-141-3p. Moreover, TGF-β actives lnc-ATB and TGF-β2 directly binds mir-141-3p. Finally, we demonstrated that lnc-ATB fulfilled its oncogenic roles in a ceRNA-mediated manner. Our study suggests that lnc-ATB promotes tumor progression by interacting with miR-141-3p and that Lnc-ATB may be a valuable prognostic predictor for GC. In conclusion, the positive feedback loop of lnc-ATB/miR-141-3p/TGF-β2 may be a potential therapeutic target for the treatment of GC.

Keywords: Gastric cancer; TGF-β; lnc-ATB; miR-141-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Proliferation*
  • Feedback, Physiological
  • Humans
  • MicroRNAs / metabolism*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Signal Transduction
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology*
  • Transforming Growth Factor beta2 / metabolism*
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • MIRN142 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • TGFB2 protein, human
  • Transforming Growth Factor beta2
  • long non-coding RNA ATB, human