MicroRNA-190b confers radio-sensitivity through negative regulation of Bcl-2 in gastric cancer cells

Biotechnol Lett. 2017 Apr;39(4):485-490. doi: 10.1007/s10529-016-2273-2. Epub 2017 Jan 2.

Abstract

Objectives: To determine the role of miR-190b in radio-sensitivity of gastric cancer (GC).

Results: In radio-resistant GC cells, down-regulation of miR-190b and up-regulation of Bcl-2 were observed. The protein expression of Bcl-2 was negatively regulated by miR-190b. Overexpression of miR-190b significantly decreased cell viability and enhanced radio-sensitivity of GC cells. Of note, these effects of miR-190b on GC cells radio-sensitivity were abolished by Bcl-2.

Conclusion: miR-190b confers radio-sensitivity of GC cells, possibly via negative regulation of Bcl-2.

Keywords: Bcl-2; Gastric cancer; MicroRNA-190b; Radio-sensitivity.

MeSH terms

  • Cell Line, Tumor
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Radiation Tolerance*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology

Substances

  • BCL2 protein, human
  • MIRN190 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins c-bcl-2