Synergisic effect of APRIL knockdown and Jiedu Xiaozheng Yin, a Chinese medicinal recipe, on the inhibition of hepatocellular carcinoma cell proliferation

Zhizhen Liu; Lianming Liao; Zhiyun Cao; Xuzheng Chen; Jian Du

doi:10.3892/or.2016.5339

Synergisic effect of APRIL knockdown and Jiedu Xiaozheng Yin, a Chinese medicinal recipe, on the inhibition of hepatocellular carcinoma cell proliferation

Oncol Rep. 2017 Feb;37(2):754-760. doi: 10.3892/or.2016.5339. Epub 2016 Dec 28.

Authors

Zhizhen Liu¹, Lianming Liao², Zhiyun Cao², Xuzheng Chen², Jian Du¹

Affiliations

¹ College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
² Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

Abstract

It is well documented that A proliferation-inducing ligand (APRIL), a member of the tumor necrosis factor superfamily, plays a crucial role in the occurrence and development of tumors. In the present study, we evaluated the synergistic effect of APRIL knockdown and Jiedu Xiaozheng Yin (JXY), a Traditional Chinese Medicinal recipe, on the inhibition of hepatocellular carcinoma (HCC) cell proliferation and elucidated the underlying mechanism. The results demonstrated that both APRIL knockdown using small interfering RNA (siRNA) and JXY treatment could trigger cell cycle arrest and cell apoptosis, and suppress HCC cell proliferation through an NF-κB-related pathway. Synergism was further demonstrated between APRIL knockdown and JXY treatment. In conclusion, these results indicate that APRIL is a target gene for HCC and combination of siRNA-APRIL and JXY application holds great promise as a novel approach for the treatment of APRIL-positive HCC.

MeSH terms

Animals
Apoptosis / drug effects*
Apoptosis / genetics
Blotting, Western
Carcinoma, Hepatocellular / drug therapy
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / pathology*
Cell Proliferation / drug effects*
Cell Proliferation / genetics
Combined Modality Therapy
Drugs, Chinese Herbal / chemistry*
Humans
Liver Neoplasms / drug therapy
Liver Neoplasms / genetics
Liver Neoplasms / pathology*
Male
Mice
Plant Extracts / pharmacology*
RNA, Messenger / genetics
RNA, Small Interfering / genetics
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Tumor Necrosis Factor Ligand Superfamily Member 13 / antagonists & inhibitors*
Tumor Necrosis Factor Ligand Superfamily Member 13 / genetics
Tumor Necrosis Factor Ligand Superfamily Member 13 / metabolism
Xenograft Model Antitumor Assays

Substances

Drugs, Chinese Herbal
Jiedu Xiaozheng Yin
Plant Extracts
RNA, Messenger
RNA, Small Interfering
Tumor Necrosis Factor Ligand Superfamily Member 13