Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression

Proc Natl Acad Sci U S A. 2017 Jan 3;114(1):101-106. doi: 10.1073/pnas.1612069113. Epub 2016 Dec 16.

Abstract

To define a complete catalog of the genes that are activated during mouse sclerotome formation, we sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. In addition to well-known early markers of sclerotome, such as Pax1, Pax9, and the Bapx2/Nkx3-2 homolog Nkx3-1, the long-noncoding RNA PEAT (Pax1 enhancer antisense transcript) was induced in sclerotome-directed samples. Strikingly, PEAT is located just upstream of the Pax1 gene. Using CRISPR/Cas9, we generated a mouse line bearing a complete deletion of the PEAT-transcribed unit. RNA-seq on PEAT mutant embryos showed that loss of PEAT modestly increases bone morphogenetic protein target gene expression and also elevates the expression of a large subset of ribosomal protein mRNAs.

Keywords: BMP pathway; CRISPR/Cas9; long-noncoding RNA; ribosomal proteins; sclerotome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bone Morphogenetic Proteins / biosynthesis
  • CRISPR-Cas Systems / genetics
  • Embryonic Development / genetics*
  • Gene Expression Regulation, Developmental / genetics*
  • Mesoderm / embryology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Paired Box Transcription Factors / biosynthesis
  • Paired Box Transcription Factors / genetics*
  • RNA, Long Noncoding / genetics*
  • RNA, Ribosomal / biosynthesis*
  • Ribosomal Proteins / biosynthesis*
  • Ribosomal Proteins / genetics
  • Sequence Deletion / genetics

Substances

  • Bone Morphogenetic Proteins
  • Paired Box Transcription Factors
  • RNA, Long Noncoding
  • RNA, Ribosomal
  • Ribosomal Proteins
  • PAX1 transcription factor