Interleukin-3 Prevents Cellular Death Induced by Oxidative Stress in HEK293 Cells

J Cell Biochem. 2017 Jun;118(6):1330-1340. doi: 10.1002/jcb.25790. Epub 2017 Jan 10.

Abstract

Interleukin-3 (IL-3) is a well-characterized growth factor in hematopoietic cells, but it is also expressed in other cell types with poorly described functions. Many studies have provided evidence that IL-3 plays an important role in cell survival. We have previously shown that IL-3 is able to increase glucose uptake in HEK293 cells, suggesting that this factor requires sustained glucose metabolism to promote cell survival. In this study, we demonstrate that IL-3 contributes to cell survival under oxidative stress, a prominent feature in the pathophysiology of cancer, diabetes, and neurodegenerative diseases, as well as in the aging process. Our results suggest a molecular mechanism that involves signaling pathways mediated by PI-3k/Akt and Erk. Altogether, these findings show an important role for IL-3 in supporting the viability of non-hematopoietic systems. J. Cell. Biochem. 118: 1330-1340, 2017. © 2016 Wiley Periodicals, Inc.

Keywords: CELL SURVIVAL; Erk; IL-3; OXIDATIVE STRESS; PI3k/Akt.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Death
  • Cell Survival / drug effects
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Glucose / metabolism*
  • HEK293 Cells
  • Humans
  • Hydrogen Peroxide / adverse effects*
  • Interleukin-3 / metabolism*
  • Oxidative Stress
  • Phosphatidylinositol 3-Kinases / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction / drug effects

Substances

  • IL3 protein, human
  • Interleukin-3
  • Hydrogen Peroxide
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins c-akt
  • Extracellular Signal-Regulated MAP Kinases
  • Glucose