Autoantibody Response to ZRF1 and KRR1 SEREX Antigens in Patients with Breast Tumors of Different Histological Types and Grades

Dis Markers. 2016:2016:5128720. doi: 10.1155/2016/5128720. Epub 2016 Oct 25.

Abstract

Purpose. To investigate a frequency of antibody response to SEREX-identified medullary breast carcinoma autoantigens ZRF1 and KRR1 in sera of breast cancer patients taking into account clinical and molecular characteristics of tumors for opening of new perspectives in creation of minimally invasive immunological tests for cancer diagnostics. Methods. Enzyme-linked immunosorbent assay and bioinformatics analysis. Results. Increased frequency of antibody response was found in sera of breast cancer patients to ZRF and KRR1 antigens. The antibody response to these antigens was higher in sera of patients with invasive ductal carcinoma than in sera of patients with other histological types of breast tumors. Moreover, more frequent antibody response to ZRF antigen was found in sera of patients with less aggressive tumors. The sequence analysis of ZRF1 antigen SEREX clones obtained from cDNA libraries of different tumors demonstrates that they encode different protein isoforms. Conclusion. Tumor-associated antigens KRR1 and ZRF1 and their cognate autoantibodies could be considered as potential molecular markers of breast cancer which need to be further investigated.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Neoplasm / blood*
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / immunology
  • Autoantibodies / blood*
  • Base Sequence
  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / immunology
  • Breast Neoplasms / blood
  • Breast Neoplasms / immunology*
  • Breast Neoplasms / pathology
  • Carcinoma, Ductal, Breast / blood
  • Carcinoma, Ductal, Breast / immunology
  • Carcinoma, Ductal, Breast / pathology
  • Carcinoma, Lobular / blood
  • Carcinoma, Lobular / immunology
  • Carcinoma, Lobular / pathology
  • Carcinoma, Medullary / blood
  • Carcinoma, Medullary / immunology
  • Carcinoma, Medullary / pathology
  • Case-Control Studies
  • DNA-Binding Proteins / blood*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Follow-Up Studies
  • Gene Library
  • Humans
  • Middle Aged
  • Molecular Chaperones
  • Neoplasm Grading
  • Neoplasm Staging
  • Nuclear Pore Complex Proteins / blood
  • Nuclear Pore Complex Proteins / genetics
  • Nuclear Pore Complex Proteins / immunology
  • Oncogene Proteins / blood*
  • Oncogene Proteins / genetics
  • Oncogene Proteins / immunology
  • Prognosis
  • RNA-Binding Proteins / blood
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / immunology
  • Young Adult

Substances

  • AGFG1 protein, human
  • Antigens, Neoplasm
  • Autoantibodies
  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • DNAJC2 protein, human
  • Molecular Chaperones
  • Nuclear Pore Complex Proteins
  • Oncogene Proteins
  • RNA-Binding Proteins