Abstract
Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases.
MeSH terms
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CRISPR-Cas Systems
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Proliferation / drug effects
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Cell Proliferation / genetics
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Cell Survival / drug effects
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Cell Survival / genetics
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Dose-Response Relationship, Drug
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HeLa Cells
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Humans
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Hydrogen Peroxide / pharmacology
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Oxidants / pharmacology
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RNA, Neoplasm / metabolism*
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Ribonuclease, Pancreatic / metabolism*
Substances
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Carrier Proteins
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Oxidants
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RNA, Neoplasm
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RNH1 protein, human
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Hydrogen Peroxide
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Ribonuclease, Pancreatic