PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas

Monoclon Antib Immunodiagn Immunother. 2016 Oct;35(5):263-266. doi: 10.1089/mab.2016.0036.

Abstract

Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.

Keywords: canine podoplanin; immunohistochemistry; monoclonal antibody.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / immunology
  • Carcinoma, Squamous Cell / pathology
  • Dogs
  • Epitopes / immunology
  • Flow Cytometry
  • Humans
  • Membrane Glycoproteins
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Mouth Neoplasms / genetics
  • Mouth Neoplasms / immunology*
  • Mouth Neoplasms / pathology

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Membrane Glycoproteins
  • Membrane Proteins
  • PDPN protein, human