Androgen induces gonadal soma-derived factor, Gsdf, in XX gonads correlated to sex-reversal but not Dmrt1 directly, in the teleost fish, northern medaka (Oryzias sakaizumii)

Yoshifumi Horie; Taijun Myosho; Tadashi Sato; Mitsuru Sakaizumi; Satoshi Hamaguchi; Tohru Kobayashi

doi:10.1016/j.mce.2016.07.022

Androgen induces gonadal soma-derived factor, Gsdf, in XX gonads correlated to sex-reversal but not Dmrt1 directly, in the teleost fish, northern medaka (Oryzias sakaizumii)

Mol Cell Endocrinol. 2016 Nov 15:436:141-9. doi: 10.1016/j.mce.2016.07.022. Epub 2016 Jul 21.

Authors

Yoshifumi Horie¹, Taijun Myosho², Tadashi Sato³, Mitsuru Sakaizumi⁴, Satoshi Hamaguchi⁵, Tohru Kobayashi⁶

Affiliations

¹ Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan. Electronic address: horie.yoshifumi@nies.go.jp.
² Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan; Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: f09m004a@alumni.niigata-u.ac.jp.
³ Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: satochu@env.sc.niigata-u.ac.jp.
⁴ Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: sakaizum@env.sc.niigata-u.ac.jp.
⁵ Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: shamaguc@env.sc.niigata-u.ac.jp.
⁶ Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan. Electronic address: tohruk@u-shizuoka-ken.ac.jp.

PMID: 27452797
DOI: 10.1016/j.mce.2016.07.022

Abstract

In the inbred HNI-II strain of Oryzias sakaizumii, Dmy and Gsdf are expressed in XY gonads from Stages 35 and 36, respectively, similarly to the inbred Hd-rR strain of Oryzias latipes. However, Dmrt1 respectively becomes detectable at Stage 36 and 5 days post hatching (dph) in the two strains. In XX HNI-II embryos, 17α-methyltestosterone (MT) induces Gsdf mRNA from Stage 36, accompanied by complete sex-reversal in all treated individuals (MT, 10 ng/mL), while Dmrt1 mRNA was first detectable at 5 dph. In XX d-rR, MT induced Gsdf mRNA expression and sex-reversal in only some of the treated individuals. Together, these results suggest the testis differentiation cascade in XY individuals differs between the HNI-II and Hd-rR strains. In addition, it is suggested that androgen-induced XX sex-reversal proceeds via an androgen-Gsdf-Dmrt1 cascade and that Gsdf plays an important role in sex-reversal in medaka.

Keywords: Androgen; Gsdf; Inbred HNI-II strain; Medaka; Oryzias sakaizumii; XX sex-reversal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

46, XX Testicular Disorders of Sex Development / metabolism*
Animals
Cell Count
Female
Fish Proteins / genetics
Fish Proteins / metabolism*
Gene Expression Regulation, Developmental / drug effects
Germ Cells / cytology
Germ Cells / drug effects
Germ Cells / metabolism
Gonads / drug effects
Gonads / metabolism*
Male
Methyltestosterone / pharmacology*
Models, Biological
Oryzias / genetics
Oryzias / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sex Determination Processes / drug effects
Sex Determination Processes / genetics
Transcription Factors / genetics
Transcription Factors / metabolism*

Substances

DMRT1 protein
Fish Proteins
RNA, Messenger
Transcription Factors
Methyltestosterone