Interaction properties of human TRAMP-like proteins and their role in pre-rRNA 5'ETS turnover

FEBS Lett. 2016 Sep;590(17):2963-72. doi: 10.1002/1873-3468.12314. Epub 2016 Aug 6.

Abstract

In yeast, the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) complex acts as a cofactor for the nuclear exosome to promote degradation of various RNAs. However, the corresponding machinery in mammals is less characterized. We analyzed the interactions of the human TRAMP-like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly. Furthermore, the human TRAMP-like proteins were involved in the RRP6-catalyzed turnover of pre-rRNA 5'ETS fragments. These results suggest the significant role for RRP6 in the assembly of TRAMP-like proteins during nucleolar RNA surveillance.

Keywords: PAP-associated domain-containing protein 5; RRP6; Trf4/5-Air1/2-Mtr4 polyadenylation; exosome; pre-rRNA; zinc finger CCHC domain-containing protein 7.

Publication types

  • Letter

MeSH terms

  • Exoribonucleases / genetics
  • Exoribonucleases / metabolism
  • Exosome Multienzyme Ribonuclease Complex / genetics
  • Exosome Multienzyme Ribonuclease Complex / metabolism
  • Humans
  • RNA Helicases / genetics
  • RNA Helicases / metabolism*
  • RNA Nucleotidyltransferases / genetics
  • RNA Nucleotidyltransferases / metabolism*
  • RNA Precursors / genetics
  • RNA Processing, Post-Transcriptional
  • RNA Stability / genetics*
  • Saccharomyces cerevisiae / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • RNA Precursors
  • Transcription Factors
  • ZCCHC7 protein, human
  • RNA Nucleotidyltransferases
  • TENT4B protein, human
  • Exoribonucleases
  • Exosome Multienzyme Ribonuclease Complex
  • EXOSC10 protein, human
  • MTREX protein, human
  • RNA Helicases